Research Tasks

UWM OLSZTYN Group

WP-1: Conducting nutritional experiments on weaners, collecting materials for research, histopathological, morphometrical, hematological, and biochemical analyzes of blood and phenotypic data. (Olsztyn)

ZUT Szczecin Group

WP-2: Characterization of the hepatotoxic effect of aflatoxin B1 (AFB1), the effect of medicinal plant extracts on liver function, and the effect of using these extracts to alleviate the toxic effect of AFB1 administration on the liver through proteome analysis. (Szczecin)

UP, LUBLIN Group

WP-3: Verification of changes in the functional state of mitochondria and changes in mitochondrial DNA in cells caused by contaminated AFB1 diet compared to a diet supplemented with hepatoprotective medicinal herb extracts (mtDNA, p53 profile, Bax, Bcl-2, cytochrome c, caspase 3, caspase 8, caspase 9, MFN2) (Lublin)

UMK, Torun Group:

WP-4: Analysis of miRNA profiling of porcine Liver using the NGS methods in piglets fed with AFB1-contaminated diet compared to feed supplemented with hepatoprotective herbs (in vivo). (Toruń)

WP-6: Validation experiments to assess changes in the expression of selected genes using the RT-PCR method in relation to the results of mRNA-seq, miRNA-seq, mtDNA, proteomics and metabolomics (Torun)

WP-9. Concerted efforts for multi-level bioinformatics analyses of integrated -Omics experimental data of WP1-WP5, WP7, and WP8 (Toruń)

PAN Jastrzebiec Group

WP-5: In vitro experiment on human and porcine immortalized hepatocytes to validate the identified key genes using siRNA. (Jastrzebiec)

WP-6: Validation experiments to assess changes in the expression of selected genes using the RT-PCR method in relation to the results of mRNA-seq, miRNA-seq, mtDNA, proteomics, and metabolomics (Jastrzebiec)

WP-9. Concerted efforts for multi-level bioinformatics analyses of integrated -Omics experimental data of WP1-WP5, WP7, and WP8 (Jastrzebiec)

FBN Dummerstorf, Germany Group

WP-7: Analysis of mRNA-seq profiling of porcine Liver using the NGS methods in piglets fed with AFB1 contaminated diet compared to feed supplemented with hepatoprotective herbs (in vivo) and in cell-cultures of human and porcine hepatocytes (in vitro) (FBN, Dummerstorf, Germany)

WP-8: Verification of metabolic changes in porcine liver and blood plasma (in vivo) and in cell cultures of human and porcine hepatocytes (in vitro) experiments (FBN, Dummerstorf, Germany)

WP-9. Concerted efforts for multi-level bioinformatics analyses of integrated -Omics experimental data of WP1-WP5, WP7, and WP8 (FBN, Dummerstorf, Germany)